MK-886, a leukotriene biosynthesis inhibitor, as an activator of Ca(2+) mobilization in Madin-Darby canine kidney (MDCK) cells.
作者： Ching-Jiunn Tseng , Chung-Ren Jan
DOI:
关键词: Endoplasmic reticulum 、 Phospholipase 、 Endocrinology 、 Chemistry 、 Phospholipase C 、 Thapsigargin 、 Aristolochic acid 、 Molecular biology 、 Phospholipase A2 、 Internal medicine 、 Phospholipase D 、 Activator (genetics)
摘要: The effect of 3-[1-( p -chlorobenzyl)-5-(isopropyl)-3- tert -butylthioindol-2-yl]-2, 2-dimethylpropanoic acid (MK-886), a leukotriene biosynthesis inhibitor, on Ca2+ mobilization in Madin- Darby canine kidney cells has been examined by fluorimetry using fura-2 as indicator. MK-886 at 0.5 to 25 μM concentration dependently increased [Ca2+]i. [Ca2+]i increase comprised an immediate initial rise and slowly decaying phase. removal inhibited the signals reducing both decay phase, suggesting that activated influx release. In Ca2+-free medium, 10 still after pretreatment with carbonylcyanide m -chlorophenylhydrazone (CCCP; 2 μM), mitochondrial uncoupler, thapsigargin (1 endoplasmic reticulum pump inhibitor. Conversely, abolished CCCP- thapsigargin-induced Ca2+release. This suggests released Ca2+from reticulum, mitochondria, other stores. addition 3 mM for 700 s indicating induced capacitative entry. entry was partly SKF96365 (50 econazole (25 inhibiting phospholipase A2with aristolochic (40 μM) but not D 0.1 propranolol. (10 μM)-induced Ca2+release altered C U73122 (2 50% suppressing A2 propranolol (0.1 mM) respectively.
参考文章(31)
C A Rouzer, A W Ford-Hutchinson, H E Morton, J W Gillard, MK886, a potent and specific leukotriene biosynthesis inhibitor blocks and reverses the membrane association of 5-lipoxygenase in ionophore-challenged leukocytes. Journal of Biological Chemistry. ,vol. 265, pp. 1436- 1442 ,(1990) , 10.1016/S0021-9258(19)40034-3
M M Billah, S Eckel, T J Mullmann, R W Egan, M I Siegel, Phosphatidylcholine Hydrolysis by Phospholipase D Determines Phosphatidate and Diglyceride Levels in Chemotactic Peptide-stimulated Human Neutrophils: Involvement of phosphatidate phosphohydrolase in signal transduction Journal of Biological Chemistry. ,vol. 264, pp. 17069- 17077 ,(1989) , 10.1016/S0021-9258(18)71460-9
A.K. Thompson, S.P. Mostafapour, L.C. Denlinger, J.E. Bleasdale, S.K. Fisher, The aminosteroid U-73122 inhibits muscarinic receptor sequestration and phosphoinositide hydrolysis in SK-N-SH neuroblastoma cells. A role for Gp in receptor compartmentation. Journal of Biological Chemistry. ,vol. 266, pp. 23856- 23862 ,(1991) , 10.1016/S0021-9258(18)54362-3
N Demaurex, D P Lew, K H Krause, Cyclopiazonic acid depletes intracellular Ca2+ stores and activates an influx pathway for divalent cations in HL-60 cells. Journal of Biological Chemistry. ,vol. 267, pp. 2318- 2324 ,(1992) , 10.1016/S0021-9258(18)45880-2
J. Gillard, A. W. Ford-Hutchinson, C. Chan, S. Charleson, D. Denis, A. Foster, R. Fortin, S. Leger, C. S. McFarlane, H. Morton, H. Piechuta, D. Riendeau, C. A. Rouzer, J. Rokach, R. Young, D. E. MacIntyre, L. Peterson, T. Bach, G. Eiermann, S. Hopple, J. Humes, L. Hupe, S. Luell, J. Metzger, R. Meurer, D. K. Miller, E. Opas, S. Pacholok, L-663,536 (MK-886) (3-[1-(4-chlorobenzyl)-3-t-butyl-thio-5-isopropylindol-2-yl]-2,2-dimethylpropanoic acid), a novel, orally active leukotriene biosynthesis inhibitor Canadian Journal of Physiology and Pharmacology. ,vol. 67, pp. 456- 464 ,(1989) , 10.1139/Y89-073
Norihiro Nagano, Yuji Imaizumi, Masaru Hirano, Minoru Watanabe, Opening of Ca2+-Dependent K+ Channels by Nordihydroguaiaretic Acid in Porcine Coronary Arterial Smooth Muscle Cells Japanese Journal of Pharmacology. ,vol. 70, pp. 281- 284 ,(1996) , 10.1254/JJP.70.281
Michael J. Berridge, Inositol trisphosphate and calcium signalling Nature. ,vol. 361, pp. 315- 325 ,(1993) , 10.1038/361315A0
Chung-Ren Jan, Chin-Man Ho, Sheng-Nan Wu, Ching-Jiunn Tseng, The phospholipase C inhibitor U73122 increases cytosolic calcium in MDCK cells by activating calcium influx and releasing stored calcium Life Sciences. ,vol. 63, pp. 895- 908 ,(1998) , 10.1016/S0024-3205(98)00346-4
Sergey V Smirnov, Greg A Knock, Philip I Aaronson, Effects of the 5‐lipoxygenase activating protein inhibitor MK886 on voltage‐gated and Ca2+‐activated K+ currents in rat arterial myocytes British Journal of Pharmacology. ,vol. 124, pp. 572- 578 ,(1998) , 10.1038/SJ.BJP.0701855
Beth S. Friedman, Elizabeth H. Bel, Agnes Buntinx, Wesley Tanaka, Yieng-Hau R. Han, Sumiko Shingo, Reynold Spector, Peter Sterk, Oral leukotriene inhibitor (MK-886) blocks allergen-induced airway responses. The American review of respiratory disease. ,vol. 147, pp. 839- 844 ,(1993) , 10.1164/AJRCCM/147.4.839