An inexpensive, automation-friendly protocol for recovering high-quality DNA
作者： NATALIA V. IVANOVA , JEREMY R. DEWAARD , PAUL D. N. HEBERT
DOI: 10.1111/J.1471-8286.2006.01428.X
关键词: Protocol (science) 、 Bioinformatics 、 Computational biology 、 DNA extraction 、 Biology 、 Microsatellite 、 Quality (business) 、 DNA 、 Automation
摘要: Although commercial kits are available for automated DNA extraction, ‘artisanal’ protocols not. In this study, we present a silica-based method that is sensitive, inexpensive and compliant with automation. The effectiveness of protocol has now been tested on more than 5000 animal specimens highly positive results.
jeremydewaard.com 参考文章(8)
M. S. Elphinstone, G. N. Hinten, M. J. Anderson, C. J. Nock, An inexpensive and high-throughput procedure to extract and purify total genomic DNA for population studies Molecular Ecology Notes. ,vol. 3, pp. 317- 320 ,(2003) , 10.1046/J.1471-8286.2003.00397.X
B. Jaulhac, M. Reyrolle, Y. K. Sodahlon, S. Jarraud, M. Kubina, H. Monteil, Y. Piémont, J. Etienne, Comparison of Sample Preparation Methods for Detection of Legionella pneumophila in Culture-Positive Bronchoalveolar Lavage Fluids by PCR Journal of Clinical Microbiology. ,vol. 36, pp. 2120- 2122 ,(1998) , 10.1128/JCM.36.7.2120-2122.1998
Mehrdad Hajibabaei, Jeremy R deWaard, Natalia V Ivanova, Sujeevan Ratnasingham, Robert T Dooh, Stephanie L Kirk, Paula M Mackie, Paul D.N Hebert, Critical factors for assembling a high volume of DNA barcodes Philosophical Transactions of the Royal Society B. ,vol. 360, pp. 1959- 1967 ,(2005) , 10.1098/RSTB.2005.1727
P. Sean Walsh, David A. Metzger, Russell Higuchi, Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material BioTechniques. ,vol. 10, pp. 506- 513 ,(2013) , 10.2144/000114018
Per Hoff-Olsen, Bente Mevåg, Eva Staalstrøm, Bente Hovde, Thore Egeland, Bjørnar Olaisen, Extraction of DNA from decomposed human tissue. An evaluation of five extraction methods for short tandem repeat typing. Forensic Science International. ,vol. 105, pp. 171- 183 ,(1999) , 10.1016/S0379-0738(99)00128-0
R Boom, C J Sol, M M Salimans, C L Jansen, P M Wertheim-van Dillen, J van der Noordaa, Rapid and simple method for purification of nucleic acids. Journal of Clinical Microbiology. ,vol. 28, pp. 495- 503 ,(1990) , 10.1128/JCM.28.3.495-503.1990
Robert D Ward, Tyler S Zemlak, Bronwyn H Innes, Peter R Last, Paul D.N Hebert, DNA barcoding Australia's fish species Philosophical Transactions of the Royal Society B: Biological Sciences. ,vol. 360, pp. 1847- 1857 ,(2005) , 10.1098/RSTB.2005.1716
Ivanova Nv, Fadeeva Gb, Rakhimova Gm, Sidorenko Sv, Degtiareva Mv, Poltaraus Ab, Sterkhova Gv, Shcherbo Sn, [Development of a PCR-based method for diagnostics of mycoses]. Molekuliarnaia genetika, mikrobiologiia i virusologiia. pp. 32- ,(2003)