Comparison of four commercial DNA extraction kits for PCR detection of Listeria monocytogenes, Salmonella, Escherichia coli O157:H7, and Staphylococcus aureus in fresh, minimally processed vegetables.
作者： P. ELIZAQUÍVEL , R. AZNAR
DOI: 10.4315/0362-028X-71.10.2110
关键词:
摘要: Four commercial DNA extraction methods, PrepMan Ultra (Applied Biosystems), InstaGene Matrix (BioRad), DNeasy Tissue kit (Qiagen), and UltraClean (MoBio), were tested for PCR detection of Listeria monocytogenes, Escherichia coli O157: H7, Salmonella, Staphylococcus aureus in fresh, minimally processed vegetables. For comparative purposes, sensitivity assays with specific PCRs carried out after the four methods green pepper, broccoli, onion artificially inoculated pathogens separately. As confirmed by statistical analysis, rendered highest values three matrices assayed L. E. O157:H7 S. aureus. Despite being most expensive compared, Kit can be successfully applied any commonly studied pathogens, thus saving time overall reducing cost analysis.
allenpress.com
jfoodprotection.com参考文章(23)
LYNETTE M. JOHNSTON, LEE-ANN JAYKUS, DEBORAH MOLL, MARTHA C. MARTINEZ, JUAN ANCISO, BRENDA MORA, CHRISTINE L. MOE, A field study of the microbiological quality of fresh produce Journal of Food Protection. ,vol. 68, pp. 1840- 1847 ,(2005) , 10.4315/0362-028X-68.9.1840
TINNA A. MANANI, ERNEST K. COLLISON, SISAI MPUCHANE, Microflora of Minimally Processed Frozen Vegetables Sold in Gaborone, Botswana Journal of Food Protection. ,vol. 69, pp. 2581- 2586 ,(2006) , 10.4315/0362-028X-69.11.2581
NATALIA V. IVANOVA, JEREMY R. DEWAARD, PAUL D. N. HEBERT, An inexpensive, automation-friendly protocol for recovering high-quality DNA Molecular Ecology Notes. ,vol. 6, pp. 998- 1002 ,(2006) , 10.1111/J.1471-8286.2006.01428.X
RICHARD L. THUNBERG, TONY T. TRAN, REGINALD W. BENNETT, ROGER N. MATTHEWS, NEGASH BELAY, Microbial evaluation of selected fresh produce obtained at retail markets. Journal of Food Protection. ,vol. 65, pp. 677- 682 ,(2002) , 10.4315/0362-028X-65.4.677
J M. Bland, D. G Altman, Multiple significance tests: the Bonferroni method BMJ. ,vol. 310, pp. 170- 170 ,(1995) , 10.1136/BMJ.310.6973.170
Sylvia Merk, Heinrich Neubauer, Hermann Meyer, Irene Greiser-Wilke, Comparison of different methods for the isolation of Burkholderia cepacia DNA from pure cultures and waste water International Journal of Hygiene and Environmental Health. ,vol. 204, pp. 127- 131 ,(2001) , 10.1078/1438-4639-00083
Ken J. Yoshitomi, Karen C. Jinneman, Stephen D. Weagant, Detection of Shiga toxin genes stx1, stx2, and the +93 uidA mutation of E. coli O157:H7/H-using SYBR Green I in a real-time multiplex PCR. Molecular and Cellular Probes. ,vol. 20, pp. 31- 41 ,(2006) , 10.1016/J.MCP.2005.09.002
Teegan Trochimchuk, John Fotheringham, Edward Topp, Heidi Schraft, Kam Tin Leung, A comparison of DNA extraction and purification methods to detect Escherichia coli O157:H7 in cattle manure. Journal of Microbiological Methods. ,vol. 54, pp. 165- 175 ,(2003) , 10.1016/S0167-7012(03)00017-4
Angela Di Pinto, VitoTony Forte, Maria Corsignano Guastadisegni, Carmela Martino, Francesco Paolo Schena, Giuseppina Tantillo, None, A comparison of DNA extraction methods for food analysis Food Control. ,vol. 18, pp. 76- 80 ,(2007) , 10.1016/J.FOODCONT.2005.08.011
G. Amagliani, C. Giammarini, E. Omiccioli, G. Brandi, M. Magnani, Detection of Listeria monocytogenes using a commercial PCR kit and different DNA extraction methods Food Control. ,vol. 18, pp. 1137- 1142 ,(2007) , 10.1016/J.FOODCONT.2006.06.012