Pre-PCR treatments as a key factor on the probability of detection of Listeria monocytogenes and Salmonella in ready-to-eat meat products by real-time PCR

摘要: Abstract The aim of this study was to compare the performance different pre-PCR treatments for detection Listeria monocytogenes and Salmonella spp. by real-time PCR in spiked ready-to-eat meat samples (cooked ham, dry-cured ham fermented sausage). Three were assayed: (i) PrepMan® Ultra Sample Preparation Reagent, (ii) DNA purification using DNeasy Tissue Kit QIAcube automated sample preparation system, (iii) BAX® system. analysis range 2 2 × 106 CFU/ml. done quintuplicate experiment repeated five times. ‘probability detection’ used efficiency calculate theoretical limit each treatment. best treatment kit QIAcube; it showed highest probability lowest food type pathogen assayed, followed PrepMan BAX treatments. Probability differences among matrices between L. monocytogenes probability. Despite being an expensive method, would be recommended reduce risk false negative results improve foodborne pathogens RTE-meat products PCR.