Cell-Kill Kinetics of Several S-Phase-specific Drugs
作者： G. L. Neil , B. K. Bhuyan , L. G. Gray , S. L. Kuentzel , T. J. Fraser
DOI:
关键词: DNA synthesis 、 Tissue culture 、 Neoplasm 、 L1210 cells 、 Thymidine 、 Camptothecin 、 Cytotoxic T cell 、 Chemotherapy 、 Biology 、 Pharmacology
摘要: Summary The drugs [1-β-d-arabinofuranosylcytosine (ara-C), hydroxyurea (HU), 5-hydroxy-2-formylpyridine thiosemicarbazone (5-HP), and camptothecin sodium salt (camptothecin)] considered in this paper markedly inhibit DNA synthesis are maximally cytotoxic to cells S phase. In these studies, high-specific-activity thymidine- 3 H (HSA-TdR- H) was used as a control compound which killed but did not affect the progression of into S. cell-kill kinetics indicated that ara-C, HU, 5-HP, unlike camptothecin, blocked L1210 from progressing presence drug. We found were G 1 by HU started moving immediately after drug removed. Therefore, time interval between two doses gave maximal cell kill same for HSA-TdR- H. However, exposed ara-C 5-HP took about 2 hr recover effect then progress (between either or 5-HP) longer than needed Camptothecin block and, therefore, with those recovery single exposure determined. correlated well required kill.
aacrjournals.org参考文章(12)
R A Tobey, A simple, rapid technique for determination of the effects of chemotherapeutic agents on mammalian cell-cycle traverse. Cancer Research. ,vol. 32, pp. 309- 316 ,(1972)
Harry A. Crissman, Robert A. Tobey, Use of Flow Microfluorometry in Detailed Analysis of Effects of Chemical Agents on Cell Cycle Progression Cancer Research. ,vol. 32, pp. 2726- 2732 ,(1972)
G L Neil, E R Homan, The Effect of Dose Interval on the Survival of L1210 Leukemic Mice Treated with DNA Synthesis Inhibitors Cancer Research. ,vol. 33, pp. 895- 901 ,(1973)
H. Madoc-Jones, F. Mauro, Age Responses of Cultured Mammalian Cells to Cytotoxic Drugs Cancer Research. ,vol. 30, pp. 1397- 1408 ,(1970)
L. G. Scheidt, B. K. Bhuyan, T. J. Fraser, Cell Cycle Phase Specificity of Antitumor Agents Cancer Research. ,vol. 32, pp. 398- 407 ,(1972)
M. H. Lindsay Gibson, Felix D. Bertalanffy, The in Vivo Effects of Arabinosylcytosine on the Cell Proliferation of Murine B16 Melanoma and Ehrlich Ascites Tumor Cancer Research. ,vol. 31, pp. 66- 71 ,(1971)
W. K. Sinclair, Hydroxyurea: Differential Lethal Effects on Cultured Mammalian Cells during the Cell Cycle Science. ,vol. 150, pp. 1729- 1731 ,(1965) , 10.1126/SCIENCE.150.3704.1729
S. Bacchetti, G. F. Whitmore, THE ACTION OF HYDROXYUREA ON MOUSE L‐CELLS* Cell Proliferation. ,vol. 2, pp. 193- 211 ,(1969) , 10.1111/J.1365-2184.1969.TB00230.X
Warren K. Sinclair, Hydroxyurea: Effects on Chinese Hamster Cells Grown in Culture Cancer Research. ,vol. 27, pp. 297- 308 ,(1967)
J. H. Kim, A. S. Gelbard, A. G. Perez, Action of Hydroxyurea on the Nucleic Acid Metabolism and Viability of HeLa Cells Cancer Research. ,vol. 27, pp. 1301- 1305 ,(1967)