Developing hypothalamic dopaminergic neurones as potential targets for environmental estrogens.

摘要: Environmental chemicals which mimic the actions of estrogen have potential to affect any responsive tissue. The aim present study was investigate their effects 17β-estradiol (E 2) on developing primary rat hypothalamic dopaminergic (DA) neurones maintained in a chemically defined medium. We now show that both E2 and octylphenol (OP), but not non-aromatizable androgen, dihydrotestosterone, enhanced uptake [ 3 H]DA by cultured cells, whereas they had no effect 14 C]GABA. Although sensitivity responses may change with age cultures, dose response curves for OP were typically ‘bell-shaped’, rise followed decline control levels increasing concentrations. Effects seen as low 10 -14 M -11 OP. Responses (10 -12 M) -9 reversed presence antiestrogen, ZM 182780 -5 M). This thus provides direct evidence, using mechanistic rather than toxicological end-point, support hypothesis inappropriate exposure environmental estrogens at critically sensitive stages development, could potentially perturb organisational activities selected neuronal populations CNS.