Proteomic changes during boron tolerance in barley(Hordeum vulgare) and the role of vacuolar proton-translocating ATPase subunit E
作者： Ahmet Emin Atik , Gönensin Ozan Bozdağ , ERSİN Akinci , Alaattin Kaya , Ahmet Koç
DOI: 10.3906/BOT-1007-29
关键词: Heterologous expression 、 Protein subunit 、 Yeast 、 RuBisCO 、 Biochemistry 、 Hordeum vulgare 、 Biology 、 Gene 、 ATPase 、 Botany 、 RNase P
摘要: Boron is an essential micronutrient for plants and animals; however, it can be toxic when present at high concentrations. The purpose of this study was to understand the mechanisms boron tolerance in Turkish barley (Hordeum vulgare) Anadolu cultivar. For purpose, 2-dimensional electrophoresis (2-DE) used screen differentially expressed proteins both control boron-stressed genotypes. Seven were revealed by 2-DE: 1) ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCo large chain), 2) TLP5, a thaumatin-like protein, 3) PR5, basic pathogenesis-related 4) RNase S-like 5) PSI type III chlorophyll a/b-binding 6) light-harvesting complex I LHC I, 7) vacuolar proton-translocating ATPase subunit E protein. These found upregulated response treatment. Even though protein encoded V-ATPase gene overexpressed, its transcript level downregulated Heterologous expression yeast provided resistance cells. results indicated that functional conferred levels might play role overall barley.
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