Control of Candida albicans Metabolism and Biofilm Formation by Pseudomonas aeruginosa Phenazines

摘要: Candida albicans has developmental programs that govern transitions between yeast and filamentous morphologies unattached biofilm lifestyles. Here, we report filamentation, intercellular adherence, development were inhibited during interactions Pseudomonas aeruginosa through the action of P. aeruginosa-produced phenazines. While phenazines are toxic to C. at millimolar concentrations, found lower concentrations any three different (pyocyanin, phenazine methosulfate, phenazine-1-carboxylate) allowed growth but affected wrinkled colony biofilms fungal yeast-to-filament transition. Phenazines impaired on nonfermentable carbon sources led increased production fermentation products (ethanol, glycerol, acetate) in glucose-containing medium, leading us propose specifically respiration. Methylene blue, another inhibitor respiration, also prevented formation structured biofilms. The inhibition filamentation wrinkling was not solely due lowered extracellular pH induced by fermentation. Compared smooth, unstructured colonies, had higher oxygen within colony, regions these colonies levels Together, our data suggest structure promotes access enhances respiratory metabolism perturbation respiration bacterial molecules such as or compounds with similar activities disrupts pathways. These findings may new ways limit context disease. IMPORTANCE Many infections caused albicans, a major human opportunistic pathogen, involve both morphological surface-associated Through study bacterium aeruginosa, which often coinfects have modulate and, metabolic effects, impact cellular morphology, cell-cell interactions, formation. We inhibits development. Our only provide insight into species valuable insights novel pathways could lead therapies treat infections.