Genotyping Mycoplasma hyorhinis by multi-locus sequence typing and multiple-locus variable-number tandem-repeat analysis.
作者： Dorottya Földi , Katinka Bekő , Orsolya Felde , Zsuzsa Kreizinger , Áron B. Kovács
DOI: 10.1016/J.VETMIC.2020.108836
关键词: Multilocus sequence typing 、 Variable number tandem repeat 、 Genetics 、 Typing 、 Locus (genetics) 、 Phylogenetic tree 、 Genotyping 、 Mycoplasma hyorhinis 、 Biology 、 Multiple Loci VNTR Analysis
摘要: Abstract Mycoplasma hyorhinis is a swine pathogen bacterium, which causes significant economic losses. The infection spreads through direct contact between the animals. Powerful genotyping methods like PCR based multi-locus sequence typing (MLST) and multiple-locus variable-number tandem-repeat analysis (MLVA) are necessary to monitor infections conduct epidemiological investigations; hence supporting control of disease. aims present study were examine M. isolates originating mainly from Hungary with MLST MLVA developed in study, compare results two methods. To characterize 39 type strain (NCTC 10,130), six house-keeping genes selected for regions chosen MLVA. We able differentiate 31 types 37 genotypes within 40 analyzed by MLVA, respectively. With combination newly assays all examined distinguished exception ones same animal. assay provided robust high resolution phylogenetic tree, while system suitable differentiation closely related farm, appropriate epidemiologic studies.
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