Investigation of Methanosarcinales and Methanomicrobiales Presence within a Dry Anaerobic Digester

摘要: Greenhouse gasses such as methane and carbon dioxide are thought to play a major role in global climate change. Anaerobic digesters function produce energy using the derived from organic matter which would typically be deposited landfills. The three main types of low-solids wet digesters, high-solids dry anaerobic digesters. Dry drastically rarer than microbial community responsible for production biogas is consequently less-studied other In this study, 16S rRNA gene copy concentrations Methanosarcinales spp. Methanomicrobiales were determined qPCR during 28-day fermentation cycle. Biogas analysis showed that fermenters with an added source, microcrystalline cellulose, produced greater volume those without substrate. At each point fermentation, present at approximately 107 genes per gram sample, whereas had between 104 106 sample. Overall, consistently more abundant every tested time point. different methanogens could indicate nutrients conditions within digester suited metabolism This information helps fill knowledge gap concerning methanogen presence used future dictate type substrate particular digester.