Assessing Methanogenic Archaeal Community in Full Scale Anaerobic Sludge Digester Systems in Dubai, United Arab Emirates
作者： Munawwar A. Khan , Poojabahen G. Patel , Arpitha G. Ganesh , Naushad Rais , Sultan M. Faheem
DOI: 10.2174/1874285801812010123
关键词:
摘要: Introduction Anaerobic digestion for methane production comprises of an exceptionally diverse microbial consortium, a profound understanding about which is still constrained. In this study, the methanogenic archaeal communities in three full-scale anaerobic digesters Municipal Wastewater Treatment Plant were analyzed by Fluorescence situ hybridization and quantitative real-time Polymerase Chain Reaction (qPCR) technique. Methods & materials (FISH) was performed to detect quantify Archaea sludge samples whereas qPCR carried out support FISH analysis. Multiple probes targeting domain archaea, different orders families used studies. Results discussion general, aceticlastic organisms (Methanosarcinaceae Methanosaetaceae) more abundant than hydrogenotrophic (Methanobacteriales, Methanomicrobiales, Methanobacteriaceae Methanococcales). Both indicated that family Methanosaetaceae most suggesting methanogenesis probably dominant pathway these digesters. Conclusion Future work involving high-throughput sequencing methods correlating with main operational parameters will help obtain better dynamics community wastewater treatment plants United Arab Emirates (UAE) turn would lead improved performance
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