Association Between Human Papillomavirus Type and Clonal Status of Cervical Squamous Intraepithelial Lesions

摘要: Background : Lesions that are histologically classified as precursors of cervical cancer, which often referred to squamous intraepithelial lesions (SILs) represent a heterogeneous clinical entity can be associated with many different types human papillomaviruses (HPVs) and have variable biologic behavior. Approximately one half low-grade SILs behave non-neoplastic, productive viral frequently regress spontaneously, whereas the other neoplasms either persist or progress higher grade lesion. Identification biomarkers reliably differentiate those properties nonneoplastic infection from neoplasia would provide more rational basis for decisions about disease management. Since monoclonality is hallmark irrespective organ site, clonal status might such biomarker. Purpose To better understand pathobiology SILs, we analyzed clonality high-grade compared their HPV types. Methods One hundred forty formalin-fixed, paraffin-embedded biopsy loop electrosurgical specimens, originally diagnosed were obtained pathology archives both Columbia-Presbyterian Medical Center Kyto Diagnostics in New York. Clonality was determined use polymerase chain reaction (PCR) based method detects nonrandom X-chromosome inactivation. This PCR-method amplifies polymorphic region androgen receptor gene flanked by several differentially methylated enzyme sites. The same tissue also DNA PCR L1 E6 consensus primers. Results All 25 evaluable cases monoclonal. Although 54 (68%) 79 monoclonal, (32%) polyclonal. A strong association observed between type status, total 71 (47 24 high-grade) monoclonal containing 16, 18, 31, 33, 35, 39, 45, 56, 58, 65. In contrast, 22 (92%) contained another polyclonal (Fisher's exact test, two-sided, P≤.001). Conclusions Our findings suggest histopathologic termed SIL consists two biologically distinct. lesion second HPV.