Unraveling the Tissue-Specific Gene Signatures of Gilthead Sea Bream (Sparus aurata L.) after Hyper- and Hypo-Osmotic Challenges.
作者： Juan Antonio Martos-Sitcha , Juan Miguel Mancera , Josep Alvar Calduch-Giner , Manuel Yúfera , Gonzalo Martínez-Rodríguez
DOI: 10.1371/JOURNAL.PONE.0148113
关键词: Gene expression 、 Internal medicine 、 Transcriptome 、 Microarray 、 Osmotic shock 、 Endocrinology 、 Gill 、 Regulation of gene expression 、 Osmotic pressure 、 Gene expression profiling 、 Cell biology 、 Biology
摘要: A custom microarray was used for the transcriptomic profiling of liver, gills and hypothalamus in response to hypo- (38‰ → 5‰) or hyper- 55‰) osmotic challenges (7 days after salinity transfer) gilthead sea bream (Sparus aurata) juveniles. The total number differentially expressed genes 777. Among them, 341 310 were liver hyper-osmotic challenges, respectively. magnitude changes lower with around 131 160 responsive at least one stress condition, Regardless tissue, a equally regulated either challenges: 127 out 524 11 19 hypothalamus. In gills, functional analysis recognized two major clusters overlapping canonical pathways that mostly related “Energy Metabolism” “Oxidative Stress”. later cluster represented all analyzed tissues, including hypothalamus, where “Cell tissue architecture” also over-represented. Overall up-regulation, whereas oxidative stress-related type highly dependent tissue. These results support common different osmoregulatory responses three helping load new allostatic conditions even return basal levels according physiological role each
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