Abnormal Patterns of Lipoprotein Lipase Release into the Plasma in GPIHBP1-deficient Mice
作者： Michael M. Weinstein , Liya Yin , Anne P. Beigneux , Brandon S. J. Davies , Peter Gin
关键词: Endocrinology 、 GPIHBP1 、 Chinese hamster ovary cell 、 Adipose tissue 、 Biology 、 Lipoprotein lipase 、 Fibrinolytic agent 、 Internal medicine 、 Heparin 、 Binding site 、 In vivo
摘要: GPIHBP1-deficient mice (Gpihbp1–/–) exhibit severe chylomicronemia. GPIHBP1 is located within capillaries of muscle and adipose tissue, expression in Chinese hamster ovary cells confers upon those the ability to bind lipoprotein lipase (LPL). However, there has been absolutely no evidence that actually interacts with LPL vivo. Heparin known release from its vivo binding sites, allowing it enter plasma. After an injection heparin, we reasoned bound would be released very quickly, hypothesized kinetics entry into plasma differ Gpihbp1–/– control mice. Indeed, levels peaked rapidly (within 1 min) after heparin In contrast, were much lower min increased slowly over 15 min. keeping result, triglycerides fell sharply 10 wild-type mice, but negligibly altered first Also, Intralipid was ineffective releasing The observed differences cannot ascribed different tissue stores LPL, as mass tissues similar intravenous strongly suggest represents important site for
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