Exploring the properties of thermostable Clostridium thermocellum cellulase CelE for the purpose of its expression in plants.
作者： RM Abdeev , IV Goldenkova , KA Musiychuk , ES Piruzian , None
关键词: Substrate specificity 、 Biochemistry 、 Cellulase 、 Chemistry 、 Bioorganic chemistry 、 Clostridium thermocellum 、 Enzyme
摘要: The main properties (pH and temperature range, stability, substrate specificity) of the modified cellulase CelE (endo-β-1,4-glucanase) from Clostridium thermocellum have been analyzed with goal its expression in plants. enzyme is similar to plant cellulases. Deletions N-terminus do not affect biochemical properties. Based on present investigation, we conclude that β-1,4-glucanase CelEM1, when expressed plants, will be a good model study role cellulases
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