HORMONALLY INDUCED MODIFICATION OF HELA ALKALINE PHOSPHATASE WITH INCREASED CATALYTIC ACTIVITY
作者： RODY P. COX , NIMAI K. GHOSH , KIRSTAN BAZZELL , MARTIN J. GRIFFIN
DOI: 10.1016/B978-0-12-472701-4.50027-9
关键词: Period (gene) 、 Protein biosynthesis 、 Kinetics 、 Biochemistry 、 Biology 、 HeLa 、 Hormone 、 Alkaline phosphatase 、 Enzyme assay 、 RNA 、 Molecular biology
摘要: ABSTRACT. Alkaline phosphatase (AP) activity of HeLa cells is increased 5 to 20 fold during growth in medium with Cortisol. The kinetics induction show a 12 hr lag period after adding hormone, followed by rapid linear increase enzyme which reaches plateau 60 90 hr. RNA and protein synthesis are required for “induction”. However, physical immunological methods that the amount AP induced not significantly increased, suggesting an enhanced catalytic efficiency responsible activity.
sci-hub.st 参考文章(28)
B.L. HORECKER, BIOCHEMISTRY OF ISOZYMES Isozymes. pp. 11- 38 ,(1975) , 10.1016/B978-0-12-472701-4.50008-5
Ronald W. Johnson, Francis T. Kenney, Regulation of Tyrosine Aminotransferase in Rat Liver Journal of Biological Chemistry. ,vol. 248, pp. 4528- 4531 ,(1973) , 10.1016/S0021-9258(19)43697-1
LEONARD S. KAPLOW, A histochemical procedure for localizing and evaluating leukocyte alkaline phosphatase activity in smears of blood and marrow. Blood. ,vol. 10, pp. 1023- 1029 ,(1955) , 10.1182/BLOOD.V10.10.1023.1023
R E Ganschow, R T Schimke, Independent Genetic Control of the Catalytic Activity and the Rate of Degradation of Catalase in Mice Journal of Biological Chemistry. ,vol. 244, pp. 4649- 4658 ,(1969) , 10.1016/S0021-9258(18)93673-2
M. J. Griffin, C. A. Lovig, R. H. Bottomley, R. Holt, Comparison of alkaline phosphatase from human normal and leukemic leukocytes. Cancer Research. ,vol. 29, pp. 1866- 1874 ,(1969)
R. L. Nath, H. N. Rydon, The influence of structure on the hydrolysis of substituted phenyl β-d-glucosides by emulsin Biochemical Journal. ,vol. 57, pp. 1- 10 ,(1954) , 10.1042/BJ0570001
Rody P. Cox, EARLY EVENTS IN HORMONAL INDUCTION OF ALKALINE PHOSPHATASE IN HUMAN CELL CULTURE: HORMONE‐CELL INTERACTION AND ITS DEPENDENCE ON DNA REPLICATION* Annals of the New York Academy of Sciences. ,vol. 179, pp. 596- 610 ,(1971) , 10.1111/J.1749-6632.1971.TB46936.X
H.M. Nitowsky, F. Herz, S. Geller, Induction of alkaline phosphatase in dispersed cell cultures by changes in osmolarity Biochemical and Biophysical Research Communications. ,vol. 12, pp. 293- 299 ,(1963) , 10.1016/0006-291X(63)90299-7
M. J. Griffin, R. P. Cox, Studies on the mechanism of hormone induction of alkaline phosphatase in human cell cultures, II. Rate of enzyme synthesis and properties of base level and induced enzymes. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 56, pp. 946- 953 ,(1966) , 10.1073/PNAS.56.3.946
M. S. Brown, A. Segal, E. R. Stadtman, Modulation of Glutamine Synthetase Adenylylation and Deadenylylation Is Mediated by Metabolic Transformation of the PII-Regulatory Protein Proceedings of the National Academy of Sciences of the United States of America. ,vol. 68, pp. 2949- 2953 ,(1971) , 10.1073/PNAS.68.12.2949