Opsonized Zymosan-Stimulated Granulocytes-Activation and Activity of the Superoxide-Generating System and Membrane Potential Changes
作者： HJ Cohen , PE Newburger , ME Chovaniec , JC Whitin , ER Simons
DOI: 10.1182/BLOOD.V58.5.975.975
关键词: Zymosan 、 Biophysics 、 Divalent 、 Depolarization 、 Biochemistry 、 Granulocyte 、 Chronic granulomatous disease 、 Stimulation 、 Membrane potential 、 Superoxide 、 Chemistry
摘要: Phagocytic cells generate superoxide in response to stimulation by opsonized particles. A continuous assay for zymosan-stimulated granulocyte production shows that there is a lag time between the addition of particles and onset detectable production. Superoxide preceded membrane potential depolarization. Neither nor depolarization occurs granulocytes from patients with chronic granulomatous disease. The extent activation zymosan affected dose 0.5 4.5 mg/ml, but necessary (lag time) not. Similarly, not attaining maximum concentration-dependent. Effects temperature, divalent cations, 2-deoxyglucose, cyanide, N-ethyl maleimide on are similar treated soluble stimuli zymosan. Thus, stimulates undergo manner elected stimuli.
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