Analysis of influenza A H3N2 strains isolated in England during 1995-1996 using polymerase chain reaction restriction.
作者： J. S. Ellis , C. J. Sadler , P. Laidler , H. Rebelo de Andrade , M. C. Zambon
DOI: 10.1002/(SICI)1096-9071(199703)51:3<234::AID-JMV16>3.0.CO;2-K
关键词: Virology 、 Endonuclease 、 Restriction map 、 Restriction enzyme 、 Virus 、 Influenza A virus 、 Orthomyxoviridae 、 Biology 、 Amplicon 、 Polymerase chain reaction
摘要: A polymerase chain reaction-restriction (PCR-restriction) endonuclease assay was developed to allow rapid analysis of influenza H3N2 viruses circulating in England during 1995-1996. Restriction digestion with two enzymes amplicons derived from PCR the HA1 portion haemagglutinin (HA) gene able differentiate antigenically similar strains into groups. Group I variants were genetically 1995/96 vaccine strain, A/Johannesburg/33/94, whereas HA sequences II reference virus A/Thessaloniki/1/95. Of 700 isolated between February 1995 and end April 1996, 384 analysed by this method. PCR-restriction sequential isolates revealed a temporal alteration prevalence variants. Groups cocirculated equal frequency period sporadic activity, but following onset epidemic activity 1995, only detected. PCR- restriction found be method for studying genetic variation which could applied large number samples provide information about direction drift virus.
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