Calcium inhibition of Physarum myosin as examined by the recombinant heavy mero-myosin.

摘要: Plasmodia of Physarum polycephalum shows vigorous cytoplasmic streaming by changing direction every few minutes. This oscillatory is regulated Ca2+ and thought to be driven a conventional myosin, i.e., myosin II isoform.1,2 While working as an assistant professor in Professor Ebashi’s laboratory at the University Tokyo, one present authors (K.K.) induced superprecipitation actomyosin preparation or B from plasmodia examine effect Ca2+. It superprecipitated without requiring When μM level was present, inhibited.3 calcium inhibition quite opposite vertebrate muscles,4 we expected that inhibitory mode could involved plant streaming.2 With finding diverse classes unconventional such I V5 muscles, shown play role cell motility both animal kingdoms. In this case myosins have calmodulin (CaM) light chains are interaction with CaM, which exerts on activity.5