Regulation by molluscan myosins.

作者: Andrew G. Szent-Györgyi , Vassilios N. Kalabokis , Cynthia L. Perreault-Micale

DOI: 10.1023/A:1006975705724

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摘要: Molluscan myosins are regulated molecules that control muscle contraction by the selective binding of calcium. The essential and regulatory light chains subunits. Scallop myosin is favorite material for studying interactions with heavy chain since can be reversibly removed from it its exchanged. Mutational structural studies show binds calcium provided Ca-binding loop stabilized specific chain. inhibitory Regulation requires presence both heads an intact headrod junction. Heavy meromyosin shows cooperative features activation while subfragment-1 non-cooperative. functionally different phasic striated smooth catch products a single gene, isoforms arise alternative splicing. differences between residues clustered at surface loop-1 account ATPase activity two types. Catch muscles contain isoforms, one phosphorylatable gizzard kinase. Phosphorylation does not alter activity. We could find evidence phosphorylation responsible state.

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