Steroids and hematopoiesis II. The effect of steroids on in vitro erythroid colony growth: Evidence for different target cells for different classes of steroids

摘要: Androgenic steroids and their non-androgenic 5beta-H metabolites enhance the number of colonies hemoglobin synthesizing cells grown from rat bone marrow in response to a standard (0.25 unit/ml) concentration erythropoietin. The target for two were found be different. Cells influenced by androgen, fluoxymesterone (fluoxy), resembled responding erythropoietin cycle characteristics, as measured tritiated thymidine suicide, physical determined velocity sedimentation gradient separation. etiocholanolone (etio) had much lower suicide rate different velocities. Preincubation with etio hours was sufficient erythroid colony growth 84%, whereas similar incubation fluoxy produced no increment. These studies demonstrate that classes may influence vitro erythropoiesis acting on distinct populations cells. Fluoxymesterone appears act through already committed respond erythropoietin, while separate, perhaps more primitive population