A mechanically improved virus-based hybrid scaffold for bone tissue regeneration

摘要: Appropriate mechanical and outstanding biological properties of biomedical scaffolds are prerequisites to successfully regenerate bone tissues. Here, we designed a hybrid scaffold consisting microsized core–sheath struts based on chemically conjugated M13 bacteriophage (phage)/alginate poly(e-caprolactone) (PCL). The filamentous phages were modified with the Arg-Gly-Asp (RGD) sequence calcium-binding sites. was mesh-like core (PCL)–sheath (phage/alginate) structure (strut size = 434 ± 51 μm, pore 495 23 completely interconnected pores). To evaluate in vitro using osteoblast-like (MG63) cells, used two controls: pure alginate RGD-modified (R-A). analyzed for various activities (tensile property, protein absorption ability, biomineralization, cell responses, osteogenic gene expression). biomineralization ability significantly higher than those R-A. Furthermore, proliferation viable cells level expression (alkaline phosphatase activity) phage/alginate enhanced compared control scaffolds. Based these results, suggest that phage/PCL-based may have potential as use tissue regeneration.