The Method Research on Tuber spp. DNA in Soil

摘要: Truffles are ascomata of ectomycorrhizal hypogeous fungi associated with endemic Pinus tabuliformis in Mount Helan National Nature Reserve Inner Mongolia, China, was investigated f soil cubes taken from pure P. stands. The objectives this study were (i) to develop a molecular method detect mycelia Tuber spp. and (ii) test for mycelial distribution around two truffle-bearing trees truffle orchard. Isolation total DNA performed, followed by PCR amplification spp.-specific primers restriction analysis. To address the detection sensitivity level, samples inoculated known amounts gleba Mycelium detected primarily within area defined burn top 35 cm all directions trees.