The occurrence of host-, pathogen- and interaction-specific proteins in the apoplast of Cladosporium fulvum (syn. Fulvia fulva) infected tomato leaves

摘要: The interaction between the intercellularly growing fungus Cladosporium fulvum and tomato was used as a model system to study accumulation of host-, pathogen- interaction-specific proteins in intercellular fluids various compatible incompatible combinations. Electrophoresis under low pH non-denaturing conditions revealed five host coded (proteins 2, 3, 4, 5 6) which accumulate 2–4- days earlier interactions than ones but litde at all healthy controls. Three these are most likely charged isomers pathogenesis-related, protein P14. In interactions, addition proteins, two others, fungal 1 7), accumulated. Protein 7 is necrosis-inducing peptide, putative product avirulence gene A9 . Proteins accumulated concomitantly with exception Cf2Cf4Cf9 /racc 2.4.5.9, where only detected. high least four (I, III, IV V) were barely detectable origin not known there indications that some origin. denaturing on SDS-polyacrylamide gels no qualitative differences profiles obtained from interactions. However, (W, X, Y P14) Western blots incubated antiserum raised against showed limited number appeared be antigenic. No additional those detected by Coomassie brilliant blue staining could immunoautoradiography.