Analysis of Defense-related Proteins in Stem Tissue of Carnation Inoculated with a Virulent and Avirulent Race of Fusarium oxysporum F.sp. Dianthi

摘要: The aim of this study was to learn more about the accumulation defense-related proteins in stem tissue from carnation cultivar Pallas inoculated with 2 near-isogenic races, avirulent race 1 and virulent 8 Fusarium oxysporum f.sp. dianthi. Stem used, which epidermis, cortex medulla were peeled off vascular cylinder. It appeared that chitinase activity constitutively expressed intercellular fluids (IFs) untreated leaves, stems roots carnation. total IFs increased time after inoculation. This increase similar inoculation virulent, water. At least four isoenzymes, three acidic one basic isoform, detected plants. In contrast, 1,3-β-glucanase not roots. Furthermore, increases markedly higher compatible incompatible interactions than water control, indicating is specially induced by elicitors common both races Using an antiserum against P3 tomato, bands on immunoblots 8. No visible Total peroxidase all combinations. One isoform present these IFs. Peroxidase a cell wall fraction prepared clearly higher, its faster, soluble fraction. These control. growth fungus Trichoderma viride inhibited obtained However, dianthi itself affected