Large-scale purification of recombinant hepatitis B surface antigen from Pichia pastoris with non-affinity chromatographic methods as a substitute to immunoaffinity chromatography.
作者： Seyed Nezamedin Hosseini , Amin Javidanbardan , Behnaz Sadat Alizadeh Salim , Maryam Khatami
DOI: 10.1080/10826068.2018.1487854
关键词: Size-exclusion chromatography 、 Hepatitis b surface antigen 、 Recombinant DNA 、 Pichia pastoris 、 Hydrophilic interaction chromatography 、 Chromatography 、 Chemistry 、 Affinity chromatography
摘要: The costly media, inconsistent ligand density, leakage, and possible destabilization of recombinant hepatitis B surface antigen (rHBsAg) particles are main drawbacks using immunoaffinity ...
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