Purification of Recombinant Hepatitis B Surface Antigen (rec-HBsAg) from P. pastoris : A Process Development Study
作者： L. Pérez , S. López , A. Beldarraín , D. Arenal , E. Pentón
DOI: 10.1007/978-94-017-0641-4_73
关键词: Ion exchange 、 HBsAg 、 Recombinant DNA 、 Hepatitis b surface antigen 、 Chemistry 、 Process development 、 Chromatography
摘要: The aim of this study was to purify rec-HBsAg cloned and expressed in P. pastoris starting from a semipurified material. A sequence six steps developed where the separation achieved by combined effect immunoaffinity, ion exchange molecular exclusion chromatographies. downstream process scaled up 250 500 fold resulted purification ≥ 97% with an overall yield 40% depending on quality characteristics
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