Exploration of inhibitory mechanisms of curcumin in lung cancer metastasis using a miRNA- transcription factor-target gene network
作者： De-min Jiao , Li Yan , Li-shan Wang , Hui-zhen Hu , Xia-li Tang
DOI: 10.1371/JOURNAL.PONE.0172470
关键词: Gene regulatory network 、 Metastasis 、 Biology 、 Lung cancer 、 Wnt signaling pathway 、 Cancer research 、 TRANSFAC 、 Transcription factor 、 Regulation of gene expression 、 microRNA 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: The present study was aimed to unravel the inhibitory mechanisms of curcumin for lung cancer metastasis via constructing a miRNA-transcription factor (TF)-target gene network. Differentially expressed miRNAs between human high-metastatic non-small cell 95D cells treated with and without were identified using TaqMan miRNA array followed by real-time PCR, out which, top 6 (miR-302b-3p, miR-335-5p, miR-338-3p, miR-34c-5p, miR-29c-3p miR-34a-35p) more verified target genes TFs than other as confirmed literature review selected further analysis. miRecords database utilized predict these miRNAs, which based on TRANSFAC database. findings above procedure used construct miRNA-TF-target network, among miR-34a-5p, miR-34c-5p miR-302b-3p seemed regulate CCND1, WNT1 MYC be involved in Wnt signaling pathway through LEF1 transcription factor. Therefore, we suggest miR-34a-5p/miR-34c-5p/miR-302b-3p -LEF1-CCND1/WNT1/MYC axis may crucial mechanism inhibition curcumin.
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