A Serum-Free Medium Developed to Support the Growth of Several Fibroblastic Cell Lines
作者： T. W. Irish , S. E. Lenk , L. A. Bugner , K. C. Torres , K. J. Etchberger
DOI: 10.1007/978-94-011-5404-8_118
关键词: Recombinant Growth Factor 、 Transformation (genetics) 、 Cell culture 、 Cell biology 、 Embryo 、 Serum free medium 、 Chemistry 、 Recombinant DNA 、 Fibroblast 、 Fibroblastic cell
摘要: A serum-free medium, EX-CELL™501, has been developed specifically to support the long term culture of several fibroblast cell lines that are capable expressing viral and protein products which also may be used in oncogenic transformation studies. During development this human derived proteins, recombinant proteins growth factors were whenever possible. We have successfully grown NIH 3T3, BALB/c Vero, BHK-21 MDBK for an extended period time (> 100 days). Final densities rates comparable those achieved using a control medium containing 10% FBS. With addition 1% FBS we able grow immortalized primary chicken embryo fibroblasts hepatocytes.
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