[6] The growth of cells in serum-free hormone-supplemented media
作者： Jane Bottenstein , Izumi Hayashi , Sharon Hutchings , Hideo Masui , Jennie Mather
DOI: 10.1016/S0076-6879(79)58127-0
关键词: Hormone 、 Serum free 、 Tissue culture 、 Trypsin inhibitor 、 Subculture (biology) 、 Biochemistry 、 Andrology 、 Trypsinization 、 Ovarian follicle 、 Chemistry 、 Cell culture
摘要: Publisher Summary This chapter presents the practical details for growth of cells in serum free hormone supplemented media. In absence serum, greater than usual care must be taken preparation synthetic portion medium. Careful water is essential consistent results with serum-free Serum and even dialyzed can mask nutritional requirements culture. Commercially available powdered media are adequate work although each batch checked suitability. also serves function a trypsin inhibitor conventional tissue culture procedures. The may necessary repair trypsinization damage after subculture; or residual left its removal, one more washes, furnishing unknown factors.
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