Distinctive DNA methylation patterns of cell-free plasma DNA in women with malignant ovarian tumors.

摘要: Abstract Objective Epithelial ovarian carcinoma (OvCa) is rarely detected early, and it also difficult to determine whether an adnexal mass benign or malignant. Previously, we noted differences in methylation patterns of cell-free plasma DNA (cfpDNA) women without disease compared patients with OvCa. In this work, investigated cfpDNA can differentiate between malignant tumors. Methods Methylation were determined three cohorts (30 samples each) using a microarray-based assay (MethDet 56). Principal component analysis, supervised clustering, linear discrimination 25 rounds 5-fold cross-validation used informative genes assess the sensitivity specificity differentiating OvCa vs. healthy control (HC), (mostly serous cystadenoma, BOD) HC, BOD samples. Results Differential promoters (RASSF1A, CALCA, EP300) differentiated HC 90.0% 86.7%. Three different (BRCA1, CDKN1C) for 76.7%. Finally, two (RASSF1A PGR-PROX) BOD, 80.0% 73.3%. Conclusions This proof-of-principle data show that differential may be useful biomarker certain Research Highlights ► from blood detect tumors These are diagnosis cancer