Vitrification of in vitro matured oocytes: effects on meiotic spindle configuration and mitochondrial function.

摘要: Background: In the assisted reproductive technique, cryopreserving in vitro-matured oocytes is a new strategy to extend pool of total oocytes. However, oocyte cryopreservation technique still unsatisfied. So assessment cyro-damage on meiotic spindle and mitochondrial function necessary evaluate refine current protocols. Material Methods: The immature were donated from women undergoing ICSI cycles. Cytoskeleton was assessed by α-tubulin mitochondria through fluorescent ΔΨm reporter JC-1. Results: Relative inner membrane potential MII vIVM group sharply decreased, compared with control (n=30) (1.397 vs. 1.019, P 0.05). Oocytes fIVM (35.5%, 11/31) (40.0%, 10/25) displayed abnormal chromosome (P>0.05). Conclusion: vitro maturation (IVM) has an adverse effect organization chromosome, no significantly discovered after vitrification-thaw cycle, while there obvious damage detected warming, which may be reason low following developmental potential.