Confocal microscopic analysis of the spindle and chromosome configurations of human oocytes matured in vitro.

摘要: Objective To assess the potential effects of in vitro maturation (IVM) human oocytes on meiotic spindle and associated chromosome configuration. Design Prospective study. Setting Hospital-based IVF center. Patient(s) Patients with polycystic ovary syndrome (PCOS) undergoing unstimulated stimulated cycles oocyte retrieval. Intervention(s) Immature (germinal vesicle metaphase I) mature (metaphase II) were collected from PCOS patients. The configurations matured vivo studied by confocal microscopy, fluorescent labeling techniques for visualization both microtubules chromatin. Main Outcome Measure(s) Meiotic configurations. Result(s) Oocytes can develop to II stage after IVM. Confocal microscopic observations revealed that had a higher frequency abnormal chromosomal alignment morphology than vivo–matured oocytes. These abnormalities included partial or total disorganization microtubules. Abnormal organization dispersal chromosomes an aberrant, less-condensed appearance. proportions abnormality 43.7% 33.3%, respectively, which was significantly those (13.6% 9.1%). Conclusion(s) In have deleterious This result suggests one possible explanation reduced developmental compared vivo. is likely contributing factor overall lower clinical outcomes observed IVM ET.