作者: T Melton , W Kundig , P E Hartman , N Meadow
DOI: 10.1128/JB.128.3.794-800.1976
关键词: PstI 、 Phosphoenolpyruvate carboxykinase 、 Biochemistry 、 Glucose transporter 、 Enzyme 、 Salmonella 、 Fructose 、 PEP group translocation 、 Mutant 、 Biology 、 Molecular biology 、 Microbiology
摘要: Three classes of phosphotransferase system mutants in Salmonella typhimurium were selected through their resistance to 3-deoxy-3-fluoro-D-glucose (DFG). Strains with mutations the ptsH (HPr) and/or pts I (enzyme I) genes on medium containing lactate plus DFG. but not pstI fructose Clones isolated from DFG semisolid plates and for ability swarm mutant either or ptsG. Mutants latter class defective enzyme IIB', a membrane component glucose transport system. Some pleiotropic properties one representative ptsG are described.