The human lens intrinsic membrane protein MP70 (Cx50) gene: clonal analysis and chromosome mapping
作者： Robert L. Church , Jianhua Wang , Ernest Steele
DOI: 10.3109/02713689509033517
关键词: Coding region 、 Genetics 、 Hybridization probe 、 genomic DNA 、 Southern blot 、 Restriction fragment 、 Chromosome 、 DNA 、 Gene 、 Biology 、 Molecular biology 、 Ophthalmology 、 Sensory Systems 、 Cellular and Molecular Neuroscience
摘要: We have isolated and characterized a human genomic clone containing the complete coding region of lens intrinsic membrane protein MP70 (Cx50). The this DNA is completely contained within one exon, as common all connexins investigated to date. size Cx50 region, from initiating ATG terminating TGA 1,299 nucleotides, for polypeptide 432 amino acids having translated molecular weight 48,171 daltons. This was used probe analyze panel Southern blots human-Chinese hamster somatic cell hybrid DNAs assign gene its chromosome. Control Chinese displayed distinct Eco R1 restriction fragment pattern when hybridized with probe. When were restricted probe, characteristic observed only chromosome 1 present in panel. Of other six connexin genes which previously been assigned chromosome, two these, Cx37 Cx40, are also found on 1.
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