Identification of lactic acid bacteria from spontaneous fermentation of 'Almagro' eggplants by SDS-PAGE whole cell protein fingerprinting.

摘要: In order to complete the previously performed phenotypic identification [Sanchez et al., 2000. Int. J. Food Microbiol. 59 (2000) 9], whole cell protein patterns obtained using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) of 149 lactic acid bacteria (LAB) strains, isolated from 'Almagro' eggplants spontaneous fermentation, were analysed. Phenotypic those strains had revealed presence species Lactobacillus (Lb) plantarum, Lb. pentosus, brevis biotype 2, 3, fermentum, spp. and Aerococcus viridans. The SDS-PAGE technique generated complex stable that easy interpret compare with 17 reference used in this study. After numerical analysis resulting electrophoretic patterns, six well-delineated clusters discriminated that, some exceptions, correlated well different groups phenotypically found. Only two showed unique without meaningful homology any for study remained unidentified.