Identification of a Tobacco mosaic virus elicitor-responsive sequence in the resistance gene N
作者： Ashraful Haque , Nobumitsu Sasaki , Hiromi Kanegae , Seisuke Mimori , Jun-Shan Gao
DOI: 10.1016/J.PMPP.2009.03.001
关键词: Elicitor 、 Tobamovirus 、 Gene expression 、 Biology 、 Transcription (biology) 、 Gene 、 Virus 、 Tobacco mosaic virus 、 Nicotiana tabacum 、 Virology 、 Molecular biology
摘要: Abstract The N gene of Nicotiana tabacum cv. Samsun NN is a resistance for Tobacco mosaic virus . transcription the increases immediately after infection or transient expression elicitor p50, helicase domain 126/180 K replicases. In this study, we cloned upstream sequences from and fused them to GFP reporter promoter assays. Agrobacterium -mediated in N. nn lacking allowed us evaluate activity with without p50 and/or Individual specifically stimulated promoter, although stimulation by was less prominent than that p50. coexpression resulted higher individual expression. Through deletion gain-of-function analyses region, identified 20-bp elicitor-responsive sequence essential sufficient Based on these data, discuss possibility may mediate cooperatively upon infection.
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