MicroRNA Regulation in Extreme Environments: Differential Expression of MicroRNAs in the Intertidal Snail Littorina littorea During Extended Periods of Freezing and Anoxia

摘要: Several recent studies of vertebrate adaptation to environmental stress have suggested roles for microRNAs (miRNAs) in regulating global suppression protein synthesis and/or restructuring expression patterns. The present study is the first characterize stress-responsive alterations miRNAs during natural freezing or anoxia exposures an invertebrate species, intertidal gastropod Littorina littorea. These snails are exposed and conditions as their environment constantly fluctuates on both a tidal seasonal basis. selected that known influence cell cycle, cellular signaling pathways, carbohydrate metabolism apoptosis was evaluated using RT-PCR. Compared controls, significant changes were observed miR-1a-1, miR-34a miR-29b hepatopancreas miR-34a, miR-133a, miR-125b, miR-2a foot muscle after exposure at -6 °C 24 h (P<0.05). In addition, response h, also miR-210 Moreover, Dicer, enzyme responsible mature microRNA processing, increased freezing. Alterations these L. littorea tissues may contribute organismal survival under anoxia.