Dehydration triggers differential microRNA expression in Xenopus laevis brain.

摘要: Abstract African clawed frogs, Xenopus laevis, although primarily aquatic, have a high tolerance for dehydration, being capable of withstanding the loss up to 32–35% total water body water. Recent studies shown that microRNAs play role in response dehydration by liver, kidney and ventral skin X. laevis. MicroRNAs act modulating expression mRNA transcripts, thereby affecting diverse biochemical pathways. In this study, 43 were assessed frog brains comparing control dehydrated (31.2 ± 0.83% lost) conditions. interest measured using modified protocol which employs polyadenylation prior reverse transcription qPCR. Twelve showed significant decrease (to 41–77% levels) from frogs (xla-miR-15a, -150, -181a, -191, -211, -218, -219b, -30c, -30e, -31, -34a, -34b) identified. Genomic analysis sequences these dehydration-responsive highly conserved as compared with comparable mice (91–100%). Suppression implies translation transcripts under their could be enhanced dehydration. Bioinformatic DIANA miRPath program (v.2.0) predicted top two KEGG pathways collectively regulate: 1. Axon guidance, 2. Long-term potentiation. Previous indicated suppression promotes neuroprotective increasing brain-derived neurotrophic factor activating anti-apoptotic This suggests similar actions may triggered laevis protective