A non-radioactive iothalamate and p-aminohippuric acid high-performance liquid chromatographic method for simultaneously measuring glomerular filtration rate and renal blood flow in the rat

摘要: A high-performance liquid chromatographic (HPLC) assay method has been developed for the quantitative determination of iothalamate and p-aminohippuric acid (PAH) concentrations in serum urine samples male rat. Glomerular filtration rate (GFR) was measured as clearance iothalamate, while effective renal blood flow (ERBF) PAH. The is simple, rapid sensitive detects PAH rat following administration bolus doses continuous infusions Samples were deproteinized with two volumes acetonitrile containing internal standard, an aliquot chromatographed on a C18 reversed-phase column. mobile phase comprised 0.1 M sodium phosphate 1.2 mM tetrabutylammonium phosphate: methanol, 85:15 (v/v), at 1.0 mL/min. analytical column eluate monitored UV detector 254 nm quantitation achieved using peak-height ratios. precision 6.6 3.6% urine, 5.6 4.9% respectively. lower limit 0.63 microgram/mL 1.25 serum, 3.1 1.5 urine. Recovery from 99.9 93.5%, 99.8 92.6%, present study demonstrated that non-radioactive can be simultaneously HPLC to measure GFR ERBF