In vivo and in vitro studies of cyclophosphamide chemotherapy in a mouse mammary carcinoma by 31P NMR spectroscopy
作者： James C. Street , Umar Mahmood , Jason A. Koutcher , Cornelia Matei
关键词: Molecular biology 、 Cell growth 、 Nuclear magnetic resonance spectroscopy 、 Cyclophosphamide 、 Biochemistry 、 Phosphocholine 、 In vivo 、 In vitro 、 Phosphocreatine 、 Chemotherapy 、 Chemistry
摘要: The effect of cyclophosphamide on the metabolic profile a mammary carcinoma implanted foot mouse was studied by 31 P NMR spectroscopy both in vivo and perchloric acid extracts. ratio nucleotide triphosphate : i significantly elevated treated tumours relative to untreated after 96 h (p < 0.05). Phosphocreatine similarly from 48 168 0.01). Resolution phosphomonoester peak into two distinct resonances allowed us estimate PME' phosphocholine (PC), where is composite consisting, part, phosphoethanolamine (PE). :PC found be higher animals control 0.01 h). Perchloric extract spectra suggest that increase PC part due decrease concentration also an previously unidentified resonance which coresonant with PE. Extract data show there significant phosphodiesters, glycerophosphocholine 0.01) glycerophosphoethanolamine 0.05) tumours. changes are qualitatively similar described following radiation they may marker cell kill or lack growth antineoplastic therapy.
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