作者: Max Larsson , Cecilie Morland , Irais Poblete-Naredo , Jürg Biber , Niels Christian Danbolt
关键词: Knockout mouse 、 Biology 、 Antiserum 、 Immunolabeling 、 Immunofluorescence 、 Nervous tissue 、 Polyclonal antibodies 、 Kidney 、 Immunohistochemistry 、 Molecular biology
摘要: The sodium-dependent inorganic phosphate transporter NaPi-IIa is expressed in the kidney. Here, authors used a polyclonal antiserum raised against NaPi-IIa- and NaPi-IIa-deficient mice to characterize its expression nervous tissue. Western blots showed that immunoreactive band (~90 kDa) was only present wild-type kidney membranes not knockout or brain membranes. In water-soluble fraction of brains, another (~50 observed; this detected Light electron microscopic immunohistochemistry using antibodies immunolabeling tubules but mice. brain, labeling presynaptic nerve terminals also This pattern produced by preimmune serum. conclude specific toward kidney, caused cross-reaction with an unknown cytosolic protein tissue-specific cross-reactivity highlights potential pitfall when validating antibody specificity mouse-derived tissue other than interest underlines utility testing sera.