Fluorescent in situ hybridization in routinely processed bone marrow aspirate clot and core biopsy sections.

摘要: Fluorescent in situ hybridization (FISH) is a technique which complements conventional cytogenetic banding analysis by allowing the evaluation of cells interphase as well metaphase. This has been used to study air-dried peripheral blood and bone marrow aspirate smears. We have applied FISH routinely processed sections clot decalcified core biopsy specimens, fixed either formalin or B5 embedded paraffin. evaluated 28 specimens (8 20 sections) for chromosome 8 copy number, studied previously cytogenetics, found following distribution: 15 with disomy, 11 trisomy, 2 tetrasomy. Using alpha-satellite probe, we detected fluorescent signals 18 (64%); 6 (75%) sections, 12 (60%) sections. Ten 13 (77%) B5-fixed (53%) formalin-fixed had hybridizing signals. Specimen age was significant factor; 10 (91%) within last months showed signals, contrast 17 (47%) older than months. In positive 200 were analyzed areas where individual could be identified. disomic two per cell seen 34 66% cells. Rare (0-2%) three detected. trisomic 19 46% tetrasomic four 25% conclude that may useful detection numerical chromosomal abnormalities such trisomy tetrasomy