No primary association of MICA polymorphism with systemic lupus erythematosus

摘要: OBJECTIVE To replicate the described association between MHC class I chain-related A (MICA) gene polymorphism and susceptibility to systemic lupus erythematosus (SLE). METHODS MICA transmembrane microsatellite was genotyped using a polymerase chain reaction (PCR)-based method. Genotyping of HLA-B* DRB1* performed PCR detection with reverse sequence-specific oligonucleotide (SSO) probe system. Combined data for these three loci (HLA-B*, MICA) were obtained from total 333 patients 361 healthy controls. RESULTS Significant B*08 [P < 10(-7), odds ratio (OR) 3.17, 95% confidence interval (CI) 2.02-5.00], DRB1*0301 (P OR 2.07, CI 1.59-2.68) MICA5.1 = 0.01, 1.23, 1.04-1.46) observed. The combinations DRB1*0301-MICA5.1-B8 HLA-DRB1*0301-B*08-positive MICA5-1-negative more frequent among SLE (11.4 vs 3.3% in controls, P 3.9 x 10(-5), 3.76, 1.85-7.73, 6.9 1.7%, 0.0007, 4.32, 1.68-13.10, respectively). Additionally, individuals who HLA-DRB1*0301-B*08-negative MICA5-1-positive less (22.2 31.3% 0.007, 0.63, 0.44-0.89) magnitude similar that negative all factors (OR 0.69, 050-0.94). Further analysis detect independent strongly suggested is secondary linkage disequilibrium this allele B*08. CONCLUSIONS Our results do not support an SLE.