Development of a highly sensitive nonisotopic immunoassay for the determination of salivary 17-hydroxyprogesterone: reference ranges throughout childhood and adolescence.

摘要: A sensitive nonisotopic immunoassay for the determination of 17-hydroxyprogesterone (17-OHP) levels in saliva was developed. The new time-resolved fluorometric employs a specific polyclonal anti-17-OHP antiserum immobilized onto microtiter plates, 17-OHP-biotin conjugate as tracer, and streptavidin-europium secondary probe. lower detection limit assay is 23.6 pmol/L (mean -3 s 22-fold zero determination) corresponding to 0.39 pg/well. coefficients intraassay variation are 8.8, 5.3, 8.3% at respective concentrations 90.9, 454.5, 1363.5 pmol/L. interassay concentrations. Saliva collected commercially available devices. Reference ranges were established using 394 samples from 132 healthy children, adolescents, adults. Morning, midday, evening 17-OHP varied significantly all age groups with morning being higher than midday levels. (n = 57) also obtained 18 children congenital adrenal hyperplasia (CAH). Salivary limited number CAH patients studied ranged 121 106 050 In conclusion 1) new, measurement has been developed; 2) reference adults have established; 3) there circadian pattern ages; 4) should be further evaluated over longer period time potentially reliable powerful technique monitor metabolic control CAH. As stable > 10 wk 4°C, ideally suited outpatient sampling.