Expression and distribution of cytokeratin 8/18 intermediate filaments in bovine antral follicles and corpus luteum: An intrinsic mechanism of resistance to apoptosis?

作者: Helen F Irving-Rodgers , Brian T Sullivan , David H Townson , Amanda N Putnam , Lankai Guo

DOI: 10.14670/HH-25.889

关键词: OvaryLuteal phaseBiologyMolecular biologyCorpus luteumEndocrinologyKeratinFollicular atresiaEstrous cycleIntermediate filamentCytokeratinInternal medicine

摘要: Apoptosis is a mechanism of cell elimination during follicular atresia and luteal regression. Recent evidence suggests sensitivity to apoptosis in some types partly dependent upon cytokeratin-containing intermediate filaments. Specifically, cytokeratin 8/18 (CK8/18) filaments are thought impart resistance apoptosis. Here, filament expression within bovine ovarian follicles corpora lutea (CL) was characterized the potential relationship between cell-specific CK8/18 explored. Immunoprecipitation western blot analysis confirmed CK8 associates with CK18 form heterodimeric cells. Immunostaining revealed populations CK18-positive (CK18+) cells healthy growing that increased postovulatory follicles. Atretic at all stages also contained CK18+ However, no were detected primordial or primary In CL, developing CL higher proportion (approximately 35%, range 30-70%) than mature 16%) regressing 5%; P<0.05, n = 3-5 CL/stage), suggesting diminishes over time, as become more susceptible Dual-fluorescence labeling for death marker (TUNEL labeling) this view, demonstrating less CK18- throughout estrous cycle (P<0.05). The results indicate differential occurs cycle. prevalence pattern these structures consistent concept might seen other types.

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