作者: Helen F Irving-Rodgers , Brian T Sullivan , David H Townson , Amanda N Putnam , Lankai Guo
DOI: 10.14670/HH-25.889
关键词: Ovary 、 Luteal phase 、 Biology 、 Molecular biology 、 Corpus luteum 、 Endocrinology 、 Keratin 、 Follicular atresia 、 Estrous cycle 、 Intermediate filament 、 Cytokeratin 、 Internal medicine
摘要: Apoptosis is a mechanism of cell elimination during follicular atresia and luteal regression. Recent evidence suggests sensitivity to apoptosis in some types partly dependent upon cytokeratin-containing intermediate filaments. Specifically, cytokeratin 8/18 (CK8/18) filaments are thought impart resistance apoptosis. Here, filament expression within bovine ovarian follicles corpora lutea (CL) was characterized the potential relationship between cell-specific CK8/18 explored. Immunoprecipitation western blot analysis confirmed CK8 associates with CK18 form heterodimeric cells. Immunostaining revealed populations CK18-positive (CK18+) cells healthy growing that increased postovulatory follicles. Atretic at all stages also contained CK18+ However, no were detected primordial or primary In CL, developing CL higher proportion (approximately 35%, range 30-70%) than mature 16%) regressing 5%; P<0.05, n = 3-5 CL/stage), suggesting diminishes over time, as become more susceptible Dual-fluorescence labeling for death marker (TUNEL labeling) this view, demonstrating less CK18- throughout estrous cycle (P<0.05). The results indicate differential occurs cycle. prevalence pattern these structures consistent concept might seen other types.