Egg output of Trichuris mûris (Schrank, 1788).

摘要: The egg output of Trichuris muris was found to reflect the stage maturity and age worms present. During 2nd week patency daily per female worm ranged from 4,000 5,000. Maximum occurred between 3rd 5th weeks patency, with a calculated range 7,500 7,900 eggs day. Late in 10, count decreased 2,100 2,400 eggs. ratio male increased as infection progressed. In early mean 1.00:1.42; terminally it 1.00 0.50 or less females. investigations bionomics studies were initiated on worms. production T. trichiura has been variously stated be 1,000 46,000 day (p. 342, Faust Russell, 1964). light present knowledge "self cure" "natural egression" at different stages preand postpatency (Campbell, 1963; Pike, Wakelin, 1967), might expected vary during course patency. This variance would depend upon changes ratio, length, duration infection. MATERIALS AND METHODS strain used kindly provided by Dr. 0. D. Standen Wellcome Laboratories, London (for origin culture see Keeling, 1961). Eggs collected feces infected mice modification zinc sulfate centrifugal-flotation technique et al. (1938, 1939). After flotation washed distilled water transferred 40-ml stender dish suspended 12 ml 0.5% formolphysiological saline solution depth 8 mm. covered and, without further aeration, left room temperature for 38 days, then stored refrigerator 4 C. Before animal inoculation cultures brought temperature; formol-saline replaced 2% tragacanth solution. Movement ovic larvae verified microscopically. placed magnetic stirrer assure homogeneity aliquot counting inoculation. volume adjusted Received publication 24 September 1968. * study supported part U. S. Public Health Service Training Grant 2E-55 (C2 C3). t Present address: Department Microbiology, New York Medical College, Avenue 106th Street, York, York. yield desired number 0.2 which given each mouse gavage. Male weanling used. C57 brown raised nematode-free colony maintained K. L. Hussey Columbia University Rockland Farms stock. The. DBA-2 author through brother-sister matings Jackson Memorial Laboratory Beginning 28 postinoculation housed individual cages hardware-cloth floors 0.25 inch base cage. Purina laboratory chow ad lib. Daily fecal examinations made thereafter indicated below. Experiment I, 14 100 Four males became patent 32 total 24-hr Stoll count. contents flasks filtered 40-mesh brass sieve recover any passed. Each divided obtained natural egression autopsy 45. At cecum large intestine removed, counted. removal living I fixed 70 C 70% alcohol measured camera lucida map measure (Rappaport Wells, 1951). II, 34 used, 17 received 150 300 6 once weekly until done observe fluctuations deposition. Mice II killed days 98 105 earlier if ceased.