Characterisation of the borgwaldt LM4E system for in vitro exposures to undiluted aerosols from next generation tobacco and nicotine products (NGPs).

摘要: Abstract Traditional in vitro exposure to combustible tobacco products utilise systems that include the use of smoking machines generate, dilute and deliver smoke cell cultures. With reported lower emissions from next generation nicotine (NGPs), including e-cigarettes heating (THPs), diluting aerosol is potentially not required. Herein we present a simplified scenario undiluted NGP aerosols, using new puffing system called LM4E. Nicotine delivery an e-cigarette was used as dosimetry marker, measured at source across 4 LM4E ports chamber. Cell viability studies, Neutral Red Uptake (NRU) assay, were performed H292 human lung epithelial cells, testing aerosols THP. E-cigarette mean generated 0.084 ± 0.005 mg/puff with no significant differences different ports, p = 0.268 (n = 10/port). Mean chamber (measured up 100 puffs) 0.046 ± 0.006 mg/puff, p = 0.061. Aerosol penetration within 55% H292 cells exposed for 2 h (240 or THP 1 h (120 puffs). There positive correlations between puff number culture media, R2 = 0.764 R2 = 0.970 NRU determined after 2 h’ resulted 21.5 ± 17.0% survival, however THP, full cytotoxicity reached 1-h exposure.