DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes.
作者： Bieong-Kil Kim , Guen-Bae Hwang , Young-Bae Seu , Jong-Soo Choi , Kyeong Sik Jin
DOI: 10.1016/J.BBAMEM.2015.06.020
关键词: Biophysics 、 Chromatography 、 Biology 、 Lamellar structure 、 Cell type 、 Transfection 、 Internalization 、 Liposome 、 Cell culture 、 Sonication 、 Zeta potential
摘要: The effects of lipid compositions on their physicochemical properties and transfection efficiencies were investigated. Four liposome formulations with different 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) to dioleoylphosphatidylethanolamine (DOPE) weight ratios investigated, that is, 1:0 (T1P0), 3:1 (T3P1), 1:1 (T1P1), 1:3 (T1P3). Mean sizes liposomes influenced by composition the preparation concentration at time sonication. Zeta potentials inversely correlated sizes. However, neither nor zeta efficiency. optimum was cell-line dependent (T1P0 T3P1 for Huh7 AGS, T1P1 COS7, T1P3 A549). shape lipoplexes changed from lamellar inverted hexagonal structure according increased ratio DOPE, but there no definite advantage specific in efficiency throughout all used cell lines. cellular internalization consistently faster T1P0, T3P1, compared lines, suggesting importance endosomal escape. Our findings show DOTAP is mainly type, not size or potential.
core.ac.uk
sci-hub.se 参考文章(49)
Yuan‐Peng Zhang, Dorothy L. Reimer, Guoyang Zhang, Patricia H. Lee, Marcel B. Bally, Self-assembling DNA-lipid particles for gene transfer. Pharmaceutical Research. ,vol. 14, pp. 190- 196 ,(1997) , 10.1023/A:1012000711033
Baichao Ma, Shubiao Zhang, Huiming Jiang, Budiao Zhao, Hongtao Lv, Lipoplex morphologies and their influences on transfection efficiency in gene delivery Journal of Controlled Release. ,vol. 123, pp. 184- 194 ,(2007) , 10.1016/J.JCONREL.2007.08.022
D. McLoughlin, R. Dias, B. Lindman, M. Cardenas, T. Nylander, K. Dawson, M. Miguel, D. Langevin, Surface complexation of DNA with insoluble monolayers. Influence of divalent counterions. Langmuir. ,vol. 21, pp. 1900- 1907 ,(2005) , 10.1021/LA047700S
Kai K. Ewert, Heather M. Evans, Alexandra Zidovska, Nathan F. Bouxsein, Ayesha Ahmad, Cyrus R. Safinya, A columnar phase of dendritic lipid-based cationic liposome-DNA complexes for gene delivery: hexagonally ordered cylindrical micelles embedded in a DNA honeycomb lattice. Journal of the American Chemical Society. ,vol. 128, pp. 3998- 4006 ,(2006) , 10.1021/JA055907H
Stefan Huebner, Bronwyn Jean Battersby, Rudo Grimm, Gregor Cevc, Lipid-DNA complex formation: reorganization and rupture of lipid vesicles in the presence of DNA as observed by cryoelectron microscopy. Biophysical Journal. ,vol. 76, pp. 3158- 3166 ,(1999) , 10.1016/S0006-3495(99)77467-9
Mohammad Ramezani, Majid Khoshhamdam, Ali Dehshahri, Bizhan Malaekeh-Nikouei, The influence of size, lipid composition and bilayer fluidity of cationic liposomes on the transfection efficiency of nanolipoplexes. Colloids and Surfaces B: Biointerfaces. ,vol. 72, pp. 1- 5 ,(2009) , 10.1016/J.COLSURFB.2009.03.018
Rumiana Koynova, Yury S. Tarahovsky, Li Wang, Robert C. MacDonald, Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA. Biochimica et Biophysica Acta. ,vol. 1768, pp. 375- 386 ,(2007) , 10.1016/J.BBAMEM.2006.10.016
Dorota Walczyk, Francesca Baldelli Bombelli, Marco P. Monopoli, Iseult Lynch, Kenneth A. Dawson, What the Cell “Sees” in Bionanoscience Journal of the American Chemical Society. ,vol. 132, pp. 5761- 5768 ,(2010) , 10.1021/JA910675V
Toon Stegmann, Jean-Yves Legendre, Gene transfer mediated by cationic lipids : lack of a correlation between lipid mixing and transfection Biochimica et Biophysica Acta. ,vol. 1325, pp. 71- 79 ,(1997) , 10.1016/S0005-2736(96)00241-6
P. L. Felgner, G. M. Ringold, Cationic liposome-mediated transfection Nature. ,vol. 337, pp. 387- 388 ,(1989) , 10.1038/337387A0