Enhanced cellular uptake and gene silencing activity of siRNA using temperature-responsive polymer-modified liposome.
作者： Jian Wang , Eri Ayano , Yoshie Maitani , Hideko Kanazawa
DOI: 10.1016/J.IJPHARM.2017.03.035
关键词:
摘要: Short interfering RNA (siRNA) delivery systems using nanoparticle carriers have been limited by inefficient intracellular delivery. One drawback is the poor cellular uptake of siRNA/particle complexes through plasma membrane and release nucleic acids into cytosol. In this study, to develop temperature-responsive liposome as a novel carrier for siRNA delivery, we prepared lipoplexes assessed gene silencing activity target genes, compared with those commercial transfection reagent, Lipofectamine RNAiMAX, non-modified or PEGylated liposomes. The polymer, N-isopropylacrylamide-co-N,N'-dimethylaminopropylacrylamide [P(NIPAAm-co-DMAPAAm)]-modified induced faster because P(NIPAAm-co-DMAPAAm) exhibits lower critical solution temperature (LCST) changing its nature from hydrophilic hydrophobic above LCST. liposomes showed significantly higher than other less cytotoxicity. Furthermore, that were internalized mainly via microtubule-dependent transport also clathrin-mediated endocytosis pathway. This first report polymer-modified thermally enhanced siRNA. dehydrated polymer on liposomes, aggregation caused around LCST, can probably be attributed effective interaction cell membrane.
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